The Binding of CD93 to Multimerin-2 Promotes Choroidal Neovascularization.

ABSTRACT:

Purpose

The purpose of this study was to investigate the involvement of CD93 and Multimerin-2 in three choroidal neovascularization (CNV) models and to evaluate their contribution in the neovascular progression of age-related macular degeneration (AMD).

Methods

Choroidal neovascular membranes collected during surgery from AMD patients were analyzed by microscopy methods. Laser-induced CNV mouse models and choroid sprouting assays (CSAs) were carried out using the CD93 knockout mouse model. An original ex vivo CSA of vascular angiogenesis, employing choroid tissues isolated from human donors, was developed.

Results

In contrast to healthy choroid endothelium, hyperproliferative choroidal endothelial cells (ECs) of AMD patients expressed high levels of CD93, and Multimer

SUBMITTER: Tosi GM 

PROVIDER: S-EPMC7425738 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature