Activation of the Erk/MAPK signaling pathway is a driver for cadmium induced prostate cancer.

ABSTRACT:

Purpose

Cadmium (Cd) is reported to be associated with carcinogenesis. The molecular mechanisms associated with Cd-induced prostate cancer (PCa) remain elusive.

Materials and methods

RWPE1, PWR1E and DU 145 cells were used. RT² Profiler Array, real-time-quantitative-PCR, immunofluorescence, cell cycle, apoptosis, proliferation and colony formation assays along with Gene Set Enrichment Analysis (GSEA) were performed.

Result

Chronic Cd exposure of non-malignant RWPE1 and PWR1E cells promoted cell survival, proliferation and colony formation with inhibition of apoptosis. Even a two-week Cd exposure of PCa cell line (DU 145) significantly increased the proliferation and decreased apoptosis. RT² profiler array of 84 genes involved in the Erk/MAPK path

SUBMITTER: Dasgupta P 

PROVIDER: S-EPMC7425797 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature