Diversity and structure of prokaryotic communities within organic and conventional farming systems in central highlands of Kenya.

ABSTRACT: Management practices such as tillage, crop rotation, irrigation, organic and inorganic inputs application are known to influence diversity and function of soil microbial populations. In this study, we investigated the effect of conventional versus organic farming systems at low and high input levels on structure and diversity of prokaryotic microbial communities. Soil samples were collected from the ongoing long-term farming system comparison trials established in 2007 at Chuka and Thika in Kenya. Physicochemical parameters for each sample were analyzed. Total DNA and RNA amplicons of variable region (V4-V7) of the 16S rRNA gene were generated on an Illumina platform using the manufacturer's instructions. Diversity indices and statistical analysis were done using QIIME2 and R packages, res

SUBMITTER: Karanja EN 

PROVIDER: S-EPMC7425915 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature