Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy.

ABSTRACT: Bacterial biofilms represent a basic form of multicellular organization that confers survival advantages to constituent cells. The sequential stages of cell ordering during biofilm development have been studied in the pathogen and model biofilm-former Vibrio cholerae It is unknown how spatial trajectories of individual cells and the collective motions of many cells drive biofilm expansion. We developed dual-view light-sheet microscopy to investigate the dynamics of biofilm development from a founder cell to a mature three-dimensional community. Tracking of individual cells revealed two distinct fates: one set of biofilm cells expanded ballistically outward, while the other became trapped at the substrate. A collective fountain-like flow transported cells to the biofilm front, bypass

SUBMITTER: Qin B 

PROVIDER: S-EPMC7426073 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature