Computational Basis for On-Demand Production of Diversified Therapeutic Phage Cocktails.

ABSTRACT: New therapies are necessary to combat increasingly antibiotic-resistant bacterial pathogens. We have developed a technology platform of computational, molecular biology, and microbiology tools which together enable on-demand production of phages that target virtually any given bacterial isolate. Two complementary computational tools that identify and precisely map prophages and other integrative genetic elements in bacterial genomes are used to identify prophage-laden bacteria that are close relatives of the target strain. Phage genomes are engineered to disable lysogeny, through use of long amplicon PCR and Gibson assembly. Finally, the engineered phage genomes are introduced into host bacteria for phage production. As an initial demonstration, we used this approach to produce a phage coc

SUBMITTER: Mageeney CM 

PROVIDER: S-EPMC7426155 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature