ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.

ABSTRACT:

Background

Somatic cell reprogramming is routinely used to generate donor-specific human induced pluripotent stem cells (hiPSCs) to facilitate studies of disease in a human context. The directed differentiation of hiPSCs can generate large quantities of patient-derived cells; however, such methodologies frequently yield heterogeneous populations of neurons and glia that require extended timelines to achieve electrophysiological maturity. More recently, transcription factor-based induction protocols have been show to rapidly generate defined neuronal populations from hiPSCs.

New method

In a manner similar to our previous adaption of NGN2-glutamatergic neuronal induction from hiPSC-derived neural progenitor cells (NPCs), we now adapt an established protocol of lentiviral overe

SUBMITTER: Barretto N 

PROVIDER: S-EPMC7426253 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature