Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus.

ABSTRACT: Despite the availability of a highly effective yellow fever virus (YFV) vaccine, outbreaks of yellow fever frequently occur in Africa and South America with significant mortality, highlighting the pressing need for antiviral drugs to manage future outbreaks. To support the discovery and development of antiviral drugs against YFV, we characterized a panel of rabbit polyclonal antibodies against the three YFV structural proteins and five non-structural proteins and demonstrated these antibody reagents in conjunction with viral RNA metabolic labeling, double-stranded RNA staining and membrane floatation assays as powerful tools for investigating YFV polyprotein processing, replication complex formation, viral RNA synthesis and high throughput discovery of antiviral drugs. Specifically, the pr

SUBMITTER: Gao Z 

PROVIDER: S-EPMC7426275 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature