Development of a novel polyprobe for simultaneous detection of six viruses infecting stone and pome fruits.

ABSTRACT: A biotin-labeled, non-isotopic, novel polyprobe was developed for the simultaneous detection of six viruses viz. apple chlorotic leaf spot virus (ACLSV), apple mosaic virus (ApMV), apple stem grooving virus (ASGV), cherry virus A (CVA), prunus necrotic ringspot virus (PNRSV) and plum pox virus (PPV) infecting stone and pome fruit trees through dot-blot hybridization assay. The sensitivity of the polyprobe was checked by serial dilutions of total RNA extracted from the tissues of infected trees. ACLSV was detected up to a dilution of 5^-5, whereas ApMV, ASGV, CVA, PPV and PNRSV up to 5^-4. The developed assay was validated following testing a total of 45 symptomatic leaf samples collected from different geographical regions of Jammu and Kashmir (India), and the presence

SUBMITTER: Noorani MS 

PROVIDER: S-EPMC7426346 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature