Real time monitoring of oxygen uptake of hepatocytes in a microreactor using optical microsensors.

ABSTRACT: Most in vitro test systems for the assessment of toxicity are based on endpoint measurements and cannot contribute much to the establishment of mechanistic models, which are crucially important for further progress in this field. Hence, in recent years, much effort has been put into the development of methods that generate kinetic data. Real time measurements of the metabolic activity of cells based on the use of oxygen sensitive microsensor beads have been shown to provide access to the mode of action of compounds in hepatocytes. However, for fully exploiting this approach a detailed knowledge of the microenvironment of the cells is required. In this work, we investigate the cellular behaviour of three types of hepatocytes, HepG2 cells, HepG2-3A4 cells and primary mouse hepatocytes, towar

SUBMITTER: Gehre C 

PROVIDER: S-EPMC7426412 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature