Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system.

ABSTRACT: Plants represent a safe and cost-effective platform for producing high-value proteins with pharmaceutical properties; however, the ability to accumulate these in commercially viable quantities is challenging. Ideal crops to serve as biofactories would include low-input, fast-growing, high-biomass species such as sugarcane. The objective of this study was to develop an efficient expression system to enable large-scale production of high-value recombinant proteins in sugarcane culms. Bovine lysozyme (BvLz) is a potent broad-spectrum antimicrobial enzyme used in the food, cosmetics and agricultural industries. Here, we report a novel strategy to achieve high-level expression of recombinant proteins using a combinatorial stacked promoter system. We demonstrate this by co-expressing BvLz under

SUBMITTER: Damaj MB 

PROVIDER: S-EPMC7426418 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature