How to Count Our Microbes? The Effect of Different Quantitative Microbiome Profiling Approaches.

ABSTRACT: Next-generation sequencing (NGS) has instigated the research on the role of the microbiome in health and disease. The compositional nature of such microbiome datasets makes it however challenging to identify those microbial taxa that are truly associated with an intervention or health outcome. Quantitative microbiome profiling overcomes the compositional structure of microbiome sequencing data by integrating absolute quantification of microbial abundances into the NGS data. Both cell-based methods (e.g., flow cytometry) and molecular methods (qPCR) have been used to determine the absolute microbial abundances, but to what extent different quantification methods generate similar quantitative microbiome profiles has so far not been explored. Here we compared relative microbiome profiling (wi

SUBMITTER: Galazzo G 

PROVIDER: S-EPMC7426659 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature