Killing Two Birds With One Stone - Strain Engineering Facilitates the Development of a Unique Rhamnolipid Production Process.

ABSTRACT: High-titer biosurfactant production in aerated fermenters using hydrophilic substrates is often hampered by excessive foaming. Ethanol has been shown to efficiently destabilize foam of rhamnolipids, a popular group of biosurfactants. To exploit this feature, we used ethanol as carbon source and defoamer, without introducing novel challenges for rhamnolipid purification. In detail, we engineered the non-pathogenic Pseudomonas putida KT2440 for heterologous rhamnolipid production from ethanol. To obtain a strain with high growth rate on ethanol as sole carbon source at elevated ethanol concentrations, adaptive laboratory evolution (ALE) was performed. Genome re-sequencing allowed to allocate the phenotypic changes to emerged mutations. Several genes were affected and differentially ex

SUBMITTER: Bator I 

PROVIDER: S-EPMC7427536 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature