Early leucine programming on protein utilization and mTOR signaling by DNA methylation in zebrafish (Danio rerio).

ABSTRACT:

Background

Early nutritional programming affects a series of metabolism, growth and development in mammals. Fish also exhibit the developmental plasticity by early nutritional programming. However, little is known about the effect of early amino acid programming on growth and metabolism.

Methods

In the present study, zebrafish (Danio rerio) was used as the experimental animal to study whether early leucine stimulation can programmatically affect the mechanistic target of rapamycin (mTOR) signaling pathway, growth and metabolism in the later life, and to undercover the mechanism of epigenetic regulation. Zebrafish larvas at 3 days post hatching (dph) were raised with 1.0% leucine from 3 to 13 dph during the critical developmental stage, then back to normal water for 70

SUBMITTER: Zhu QS 

PROVIDER: S-EPMC7427859 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature