Disruption of pyruvate phosphate dikinase in Brucella ovis PA CO₂-dependent and independent strains generates attenuation in the mouse model.

ABSTRACT: Brucella ovis is a non-zoonotic rough Brucella that causes genital lesions, abortions and increased perinatal mortality in sheep and is responsible for important economic losses worldwide. Research on virulence factors of B. ovis is necessary for deciphering the mechanisms that enable this facultative intracellular pathogen to establish persistent infections and for developing a species-specific vaccine, a need in areas where the cross-protecting ovine smooth B. melitensis Rev1 vaccine is banned. Although several B. ovis virulence factors have been identified, there is little information on its metabolic abilities and their role in virulence. Here, we report that deletion of pyruvate phosphate dikinase (PpdK, catalyzing the bidirectional conversion pyruvate ⇌ phosphoenolpyruvate) in B. ovi

SUBMITTER: Vizcaino N 

PROVIDER: S-EPMC7427901 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature