Optical control of sphingosine-1-phosphate formation and function.

ABSTRACT: Sphingosine-1-phosphate (S1P) plays important roles as a signaling lipid in a variety of physiological and pathophysiological processes. S1P signals via a family of G-protein-coupled receptors (GPCRs) (S1P_1-5) and intracellular targets. Here, we report on photoswitchable analogs of S1P and its precursor sphingosine, respectively termed PhotoS1P and PhotoSph. PhotoS1P enables optical control of S1P_1-3, shown through electrophysiology and Ca²⁺ mobilization assays. We evaluated PhotoS1P in vivo, where it reversibly controlled S1P₃-dependent pain hypersensitivity in mice. The hypersensitivity induced by PhotoS1P is comparable to that induced by S1P. PhotoS1P is uniquely suited for the study of S1P biology in cultured cells and in vivo because it exhi

SUBMITTER: Morstein J 

PROVIDER: S-EPMC7428055 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature