High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay.

ABSTRACT:

Objective

Serological testing is needed to investigate the extent of transmission of SARS-CoV-2 from front-line essential workers to their household members. However, the requirement for serum/plasma limits serological testing to clinical settings where it is feasible to collect and process venous blood. To address this problem we developed a serological test for SARS-CoV-2 IgG antibodies that requires only a single drop of finger stick capillary whole blood, collected in the home and dried on filter paper (dried blood spot, DBS). We describe assay performance and demonstrate its utility for remote sampling with results from a community-based study.

Methods

An ELISA to the receptor binding domain of the SARS-CoV-2 spike protein was optimized to quantify IgG antibodies in DBS

SUBMITTER: McDade TW 

PROVIDER: S-EPMC7428174 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature