Altered 3D chromatin structure permits inversional recombination at the IgH locus.

ABSTRACT: Immunoglobulin heavy chain (IgH) genes are assembled by two sequential DNA rearrangement events that are initiated by recombination activating gene products (RAG) 1 and 2. Diversity (D_H) gene segments rearrange first, followed by variable (V_H) gene rearrangements. Here, we provide evidence that each rearrangement step is guided by different rules of engagement between rearranging gene segments. D_H gene segments, which recombine by deletion of intervening DNA, must be located within a RAG1/2 scanning domain for efficient recombination. In the absence of intergenic control region 1, a regulatory sequence that delineates the RAG scanning domain on wild-type IgH alleles, V_H and D_H gene segments can recombine with each other b

SUBMITTER: Qiu X 

PROVIDER: S-EPMC7428332 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature