Differential localization of Hessian fly candidate effectors in resistant and susceptible wheat plants.

ABSTRACT: Hessian fly Mayetiola destructor is a notorious pest of wheat. Previous studies suggest that Hessian fly uses effector-based mechanisms to attack wheat plants during parasitism, but no direct evidence has been reported to support this postulation. Here, we produced recombinant proteins for five Family-1 candidate effectors and antibodies. Indirect immunostaining and western blots were carried out to examine the localization of Hessian fly Family-1 proteins in plant and insect tissues. Confocal images revealed that Family-1 putative effectors were exclusively produced in the basal region of larval salivary glands, which are directly linked to the mandibles' ducts for effector injection. The five Family-1 proteins were detected in infested host plants on western blots. Indirect immuno

SUBMITTER: Aljbory Z 

PROVIDER: S-EPMC7428492 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature