Accelerating gene function discovery by rapid phenotyping of fatty acid composition and oil content of single transgenic T₁ Arabidopsis and camelina seeds.

ABSTRACT: Arabidopsis is wildly used as a model plant and camelina is increasingly used for oilseed research and applications. Although the Arabidopsis genome has been sequenced for two decades, the functions of many lipid-related genes and their regulators have not been well characterized. Improvements in the efficiency and accuracy of gene investigations are key to effective discovery of gene function and downstream bioengineering of plant oil quantity and quality. In this study, a visible marker was used to quickly identify transgenic T₁ seeds and a method has been developed to phenotype fatty acid compositions and oil content of single T₁ seeds. A whole seed direct transmethylation method was first optimized with multiple seeds and incubation at 85°C for 2 hours in a transm

SUBMITTER: Ma S 

PROVIDER: S-EPMC7428496 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature