Comparative Proteomic Profiling of Unannotated Microproteins and Alternative Proteins in Human Cell Lines.

ABSTRACT: Ribosome profiling and mass spectrometry have revealed thousands of small and alternative open reading frames (sm/alt-ORFs) that are translated into polypeptides variously termed as microproteins and alt-proteins in mammalian cells. Some micro-/alt-proteins exhibit stress-, cell-type-, and/or tissue-specific expression; understanding this regulated expression will be critical to elucidating their functions. While differential translation has been inferred by ribosome profiling, quantitative mass spectrometry-based proteomics is needed for direct comparison of microprotein and alt-protein expression between samples and conditions. However, while label-free quantitative proteomics has been applied to detect stress-dependent expression of bacterial microproteins, this approach has not yet bee

SUBMITTER: Cao X 

PROVIDER: S-EPMC7429271 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature