Molecular characterization of the 2018 outbreak of lumpy skin disease in cattle in Upper Egypt.

ABSTRACT:

Background and aim

Lumpy skin disease (LSD), an infectious disease of cattle, is characterized by raised nodules on the skin. Although the morbidity rate of LSD is low, it has a considerable fatality rate. Despite the annual mass vaccination of livestock with sheep pox vaccine (Veterinary Serum and Vaccine Research Institute, Egypt) enforced by Egyptian authorities, the LSD virus (LSDV) continues to circulate almost every summer. The present study aimed to discover the cause of cows naturally infected with LSDV circulating in Upper Egypt during the summer of 2018 using polymerase chain reaction (PCR) assay and to analyze their phylogenetics against reference genome sequences.

Materials and methods

We cultured LSDV in specific pathogen-free embryonated chicken eggs (SPF-ECE)

SUBMITTER: Allam AM 

PROVIDER: S-EPMC7429391 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature