Computational-based insights into the phylogeny, structure, and function of Rhodococcus alkane-1-monooxygenase.

ABSTRACT: Alkane-1-monooxygenase of alkanotrophic Rhodococcus species has been characterized using standard bioinformatics tools to investigate phylogenetic relationships, and three-dimensional structure and functions. Results revealed that activity of the Rhodococcus alkane-1-monooxygenase would be optimum in alkaline pH as their isoelectric points were in the range of 7.5 to 9. Higher aliphatic index (87 to 95) indicated that these enzymes are thermostable. Extinction coefficient of the enzyme varied from 68,793 to 1,25,820 M^-1 cm^-1 and average molecular weight was 45 kDa. Secondary structures predicted maximum alpha-helical content rather than the other conformations such as sheets or turns. The instability index (II) of most stable query protein was 39.7% whic

SUBMITTER: Pal S 

PROVIDER: S-EPMC7429611 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature