TMEM175 mediates Lysosomal function and participates in neuronal injury induced by cerebral ischemia-reperfusion.

ABSTRACT: As the main organelles for the clearance of damaged proteins and damaged organelles, the function of lysosomes is crucial for maintaining the intracellular homeostasis of long-lived neurons. A stable acidic environment is essential for lysosomes to perform their functions. TMEM175 has been identified as a new K⁺ channel that is responsible for regulating lysosomal membrane potential and pH stability in neurons. This study aimed to understand the role of TMEM175 in lysosomal function of neurons and neuronal injury following cerebral ischemia-reperfusion (I/R). A middle-cerebral-artery occlusion/reperfusion (MCAO/R) model was established in adult male Sprague-Dawley rats in vivo, and cultured neurons were exposed to oxygen-glucose deprivation/reoxygenation (OGD/R) to mimic ischemi

SUBMITTER: Zhang M 

PROVIDER: S-EPMC7429711 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature