Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells.

ABSTRACT:

Background

Although cord blood (CB) offers promise for treatment of patients with high-risk hematological malignancies and immune disorders, the limited numbers of hematopoietic stem cell (HSC)/progenitor cell in a CB unit and straitened circumstances in expanding ex vivo make it quite challenging to develop the successful cell therapies.

Methods

In this study, a novel strategy has been developed to support ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs) by coculture with engineered human umbilical arterial endothelial cells (HuAECs-E4orf1-GFP), which expresses E4ORF1 stably by using a retroviral system.

Results

Coculture of CD34⁺ hCB cells with HuAECs-E4orf1-GFP resulted in generation of considerably more total nucleated cells, CD34

SUBMITTER: Li H 

PROVIDER: S-EPMC7429738 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature