Transient exposure to miR-203 enhances the differentiation capacity of established pluripotent stem cells.

ABSTRACT: Full differentiation potential along with self-renewal capacity is a major property of pluripotent stem cells (PSCs). However, the differentiation capacity frequently decreases during expansion of PSCs in vitro. We show here that transient exposure to a single microRNA, expressed at early stages during normal development, improves the differentiation capacity of already-established murine and human PSCs. Short exposure to miR-203 in PSCs (miPSCs) induces a transient expression of 2C markers that later results in expanded differentiation potency to multiple lineages, as well as improved efficiency in tetraploid complementation and human-mouse interspecies chimerism assays. Mechanistically, these effects are at least partially mediated by direct repression of de novo DNA methyltransferases D

SUBMITTER: Salazar-Roa M 

PROVIDER: S-EPMC7429746 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature