Kinetics of T lymphocyte subsets and B lymphocytes in response to immunostimulants in flounder (Paralichthys olivaceus): implications for CD4⁺ T lymphocyte differentiation.

ABSTRACT: CD4⁺ T lymphocytes play crucial roles in the adaptive immune system. CD4, as the most effective marker to delineate the T-helper subsets, was identified in many fish species. Two CD4 homologs, CD4-1 and CD4-2, have been reported in flounder (Paralichthys olivaceus). In this study, monoclonal antibodies (mAbs) against CD4-1 and CD4-2 of flounder were produced, CD4⁺ T lymphocytes were isolated and identified, and the variations in CD4⁺ and CD8⁺ T lymphocytes and IgM⁺ B lymphocytes after Poly I:C, PMA or β-glucan stimulation were investigated. Then, the expression of transcription factors and cytokines in sorted CD4⁺ T lymphocytes was analyzed. The results showed that the mAbs were specific to flounder CD4-1⁺ and CD4

SUBMITTER: Xing J 

PROVIDER: S-EPMC7429840 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature