Isoforms of RNF128 Regulate the Stability of Mutant P53 in Barrett's Esophageal Cells.

ABSTRACT:

Background & aims

Barrett's esophagus (BE) can progress to dysplasia and esophageal adenocarcinoma (EAC), accompanied by mutations in TP53 that increase the stability of its product, p53. We analyzed BE tissues for messenger RNAs (mRNAs) that associate with BE progression and identified one that affects the stabilization of p53.

Methods

We obtained 54 BE samples collected from patients with high-grade dysplasia (HGD) or esophageal adenocarcinoma (EAC), from 1992 through 2015, and performed RNA sequence analyses, including isoform-specific analyses. We performed reverse-transcription polymerase chain reaction analyses of 166 samples and immunohistochemical analyses of tissue microarrays that contained BE tissues from 100 patients with HGD or EAC and normal esophageal squamous

SUBMITTER: Ray D 

PROVIDER: S-EPMC7429981 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature