Identification and catalytic properties of new epoxide hydrolases from the genomic data of soil bacteria.

ABSTRACT: Epoxide hydrolases (EHs) catalyse the conversion of epoxides into vicinal diols. These enzymes have extensive value in biocatalysis as they can generate enantiopure epoxides and diols which are important and versatile synthetic intermediates for the fine chemical and pharmaceutical industries. Despite these benefits, they have seen limited use in the bioindustry and novel EHs continue to be reported in the literature. We identified twenty-nine putative EHs within the genomes of soil bacteria. Eight of these EHs were explored in terms of their activity. Two limonene epoxide hydrolases (LEHs) and one ⍺/β EH were active on a model compound styrene oxide and its ring-substituted derivatives, with low to good percentage conversions of 18-86%. Further exploration of the substrate scope with enan

SUBMITTER: Stojanovski G 

PROVIDER: S-EPMC7429986 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature