A novel culture method that sustains ERα signaling in human breast cancer tissue microstructures.

ABSTRACT:

Background

Estrogen receptor α (ERα) signaling is a defining and driving event in most breast cancers; ERα is detected in malignant epithelial cells of 75% of all breast cancers (classified as ER-positive breast cancer) and, in these cases, ERα targeting is the main therapeutic strategy. However, the biological determinants of ERα heterogeneity and the mechanisms underlying therapeutic resistance are still elusive, hampered by the challenges in developing experimental models recapitulative of intra-tumoral heterogeneity and in which ERα signaling is sustained. Ex vivo cultures of human breast cancer tissue have been proposed to retain the original tissue architecture, epithelial and stromal cell components and ERα. However, loss of cellularity, viability and ERα expression are well

SUBMITTER: Cartaxo AL 

PROVIDER: S-EPMC7430012 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature