Achieving pure spin effects by artifact suppression in methyl adiabatic relaxation experiments.

ABSTRACT: Recent methyl adiabatic relaxation dispersion experiments provide examination of conformational dynamics across a very wide timescale (10²-10⁵ s^-1) and, particularly, provide insight into the hydrophobic core of proteins and allosteric effects associated with modulators. The experiments require efficient decoupling of ¹H and ¹³C spin interactions, and some artifacts have been discovered, which are associated with the design of the proton decoupling scheme. The experimental data suggest that the original design is valid; however, pulse sequences with either no proton decoupling or proton decoupling with imperfect pulses can potentially exhibit complications in the experiments. Here, we demonstrate that pulse imperfections in the proton

SUBMITTER: Chao FA 

PROVIDER: S-EPMC7430055 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature