Processing by RNase 1 forms tRNA halves and distinct Y RNA fragments in the extracellular environment.

ABSTRACT: Extracellular RNAs participate in intercellular communication, and are being studied as promising minimally invasive diagnostic markers. Several studies in recent years showed that tRNA halves and distinct Y RNA fragments are abundant in the extracellular space, including in biofluids. While their regulatory and diagnostic potential has gained a substantial amount of attention, the biogenesis of these extracellular RNA fragments remains largely unexplored. Here, we demonstrate that these fragments are produced by RNase 1, a highly active secreted nuclease. We use RNA sequencing to investigate the effect of a null mutation of RNase 1 on the levels of tRNA halves and Y RNA fragments in the extracellular environment of cultured human cells. We complement and extend our RNA sequencing results

SUBMITTER: Nechooshtan G 

PROVIDER: S-EPMC7430647 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature