TRNA elbow modifications affect the tRNA pseudouridine synthase TruB and the methyltransferase TrmA.

ABSTRACT: tRNAs constitute the most highly modified class of RNA. Every tRNA contains a unique set of modifications, and Ψ55, m⁵U54, and m⁷G46 are frequently found within the elbow of the tRNA structure. Despite the abundance of tRNA modifications, we are only beginning to understand the orchestration of modification enzymes during tRNA maturation. Here, we investigated whether pre-existing modifications impact the binding affinity or catalysis by tRNA elbow modification enzymes. Specifically, we focused on the Escherichia coli enzymes TruB, TrmA, and TrmB which generate Ψ55, m⁵U54, and m⁷G46, respectively. tRNAs containing a single modification were prepared, and the binding and activity preferences of purified E. coli TrmA, TruB, and TrmB w

SUBMITTER: Schultz SK 

PROVIDER: S-EPMC7430675 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature