Myoviridae phage PDX kills enteroaggregative Escherichia coli without human microbiome dysbiosis.

ABSTRACT: Introduction. Bacteriophage therapy can be developed to target emerging diarrhoeal pathogens, but doing so in the absence of microbiome disruption, which occurs with antibiotic treatment, has not been established.Aim. Identify a therapeutic bacteriophage that kills diarrhoeagenic enteroaggregative Escherichia coli (EAEC) while leaving the human microbiome intact.Methodology. Phages from wastewater in Portland, OR, USA were screened for bacteriolytic activity by overlay assay. One isolated phage, PDX, was classified by electron microscopy and genome sequencing. A mouse model of infection determined whether the phage was therapeutic against EAEC. 16S metagenomic analysis of anaerobic cultures determined whether a normal human microbiome was altered by treat

SUBMITTER: Cepko LCS 

PROVIDER: S-EPMC7431101 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature