ENST00000430471 Promotes Development and Metastasis of Colorectal Cancer by Regulating the Expression of YBX-1.

ABSTRACT:

Background information

Colorectal cancer (CRC) is a common malignant tumor of the digestive system. Long non-coding (lnc) RNA ENST00000430471 has been reported to be involved in CRC development and metastasis because of its cancer-promoting ability. However, the detailed molecular mechanisms of ENST00000430471 in CRC remain largely unknown.

Methods

The cell proliferation assay and Xenograft experiment were performed to examine the proliferation rate of the tumor cells. Invasiveness and migration capability of the cells were evaluated using the invasion and wound healing assays, respectively. In addition, the RNA pull-down assay and subsequent mass spectrometry techniques were performed to identify the proteins interacting with ENST00000430471.

Results

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC7431171 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature