High-Resolution Diffusion Measurements of Proteins by NMR under Near-Physiological Conditions.

ABSTRACT: Measuring the translational diffusion of proteins under physiological conditions can be very informative, especially when multiple diffusing species can be distinguished. Diffusion NMR or diffusion-ordered spectroscopy (DOSY) is widely used to study molecular diffusion, where protons are used as probes, which can be further edited by the proton-attached heteronuclei to provide additional resolution. For example, the combination of the backbone amide protons (¹H^N) to measure diffusion with the well-resolved ¹H/¹⁵N correlations has afforded high-resolution DOSY experiments. However, significant amide-water proton exchange at physiological temperature and pH can affect the accuracy of diffusion data or cause complete loss of DOSY signals. Although a

SUBMITTER: Lee J 

PROVIDER: S-EPMC7431297 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature