M⁶A Reader: Epitranscriptome Target Prediction and Functional Characterization of N ⁶-Methyladenosine (m⁶A) Readers.

ABSTRACT: N ⁶-methyladenosine (m⁶A) is the most abundant post-transcriptional modification in mRNA, and regulates critical biological functions via m⁶A reader proteins that bind to m⁶A-containing transcripts. There exist multiple m⁶A reader proteins in the human genome, but their respective binding specificity and functional relevance under different biological contexts are not yet fully understood due to the limitation of experimental approaches. An in silico study was devised to unveil the target specificity and regulatory functions of different m⁶A readers. We established a support vector machine-based computational framework to predict the epitranscriptome-wide targets of six m⁶A reader proteins (YTHDF1-3,

SUBMITTER: Zhen D 

PROVIDER: S-EPMC7431669 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature