Residues within the Foot-and-Mouth Disease Virus 3D^pol Nuclear Localization Signal Affect Polymerase Fidelity.

ABSTRACT: Many RNA viruses encode a proof-reading deficient, low-fidelity RNA-dependent polymerase (RdRp), which generates genetically diverse populations that can adapt to changing environments and thwart antiviral therapies. 3D^pol, the RdRp of the foot-and-mouth disease virus (FMDV), is responsible for replication of viral genomes. The 3D^pol N terminus encodes a nuclear localization signal (NLS) sequence,MRKTKLAPT, important for import of the protein to host nucleus. Previous studies showed that substitutions at residues 18 and 20 of the NLS are defective in proper incorporation of nucleotides and RNA binding. Here, we use a systematic alanine scanning mutagenesis approach to understand the role of individual residues of the NLS in nuclear localization and nucleotide incorpor

SUBMITTER: Kloc A 

PROVIDER: S-EPMC7431809 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature