Transformation of Riccia fluitans, an Amphibious Liverwort Dynamically Responding to Environmental Changes.

ABSTRACT: The colonization of land by streptophyte algae, ancestors of embryophyte plants, was a fundamental event in the history of life on earth. Bryophytes are early diversifying land plants that mark the transition from freshwater to terrestrial ecosystems. The amphibious liverwort Riccia fluitans can thrive in aquatic and terrestrial environments and thus represents an ideal organism to investigate this major transition. Therefore, we aimed to establish a transformation protocol for R. fluitans to make it amenable for genetic analyses. An Agrobacterium transformation procedure using R. fluitans callus tissue allows to generate stably transformed plants within 10 weeks. Furthermore, for comprehensive studies spanning all life stages, we demonstrate that the switch from vegetative to reproductive

SUBMITTER: Althoff F 

PROVIDER: S-EPMC7432341 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature