Satellite cell-specific ablation of Cdon impairs integrin activation, FGF signalling, and muscle regeneration.

ABSTRACT:

Background

Perturbation in cell adhesion and growth factor signalling in satellite cells results in decreased muscle regenerative capacity. Cdon (also called Cdo) is a component of cell adhesion complexes implicated in myogenic differentiation, but its role in muscle regeneration remains to be determined.

Methods

We generated inducible satellite cell-specific Cdon ablation in mice by utilizing a conditional Cdon allele and Pax7 ^CreERT2 . To induce Cdon ablation, mice were intraperitoneally injected with tamoxifen (tmx). Using cardiotoxin-induced muscle injury, the effect of Cdon depletion on satellite cell function was examined by histochemistry, immunostaining, and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay. Isolated myofibers or myoblasts were utilized

SUBMITTER: Bae JH 

PROVIDER: S-EPMC7432598 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature