Investigation of Strand-Selective Interaction of SNA-Modified siRNA with AGO2-MID.

ABSTRACT: Small interfering RNA (siRNA) has been recognized as a powerful gene-silencing tool. For therapeutic application, chemical modification is often required to improve the properties of siRNA, including its nuclease resistance, activity, off-target effects, and tissue distribution. Careful siRNA guide strand selection in the RNA-induced silencing complex (RISC) is important to increase the RNA interference (RNAi) activity as well as to reduce off-target effects. The passenger strand-mediated off-target activity was previously reduced and on-target activity was enhanced by substitution with acyclic artificial nucleic acid, namely serinol nucleic acid (SNA). In the present study, the reduction of off-target activity caused by the passenger strand was investigated by modifying siRNAs with SNA. T

SUBMITTER: Kamiya Y 

PROVIDER: S-EPMC7432901 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature