Dynamic nanopore long-read sequencing analysis of HIV-1 splicing events during the early steps of infection.

ABSTRACT:

Background

Alternative splicing is a key step in Human Immunodeficiency Virus type 1 (HIV-1) replication that is tightly regulated both temporally and spatially. More than 50 different transcripts can be generated from a single HIV-1 unspliced pre-messenger RNA (pre-mRNA) and a balanced proportion of unspliced and spliced transcripts is critical for the production of infectious virions. Understanding the mechanisms involved in the regulation of viral RNA is therefore of potential therapeutic interest. However, monitoring the regulation of alternative splicing events at a transcriptome-wide level during cell infection is challenging. Here we used the long-read cDNA sequencing developed by Oxford Nanopore Technologies (ONT) to explore in a quantitative manner the complexity of the HI

SUBMITTER: Nguyen Quang N 

PROVIDER: S-EPMC7433067 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature