Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells.

ABSTRACT:

Background

Syndecans regulate cell migration thus having key roles in scarring and wound healing processes. Our previous results have shown that Thy-1/CD90 can engage both αvβ3 integrin and Syndecan-4 expressed on the surface of astrocytes to induce cell migration. Despite a well-described role of Syndecan-4 during cell movement, information is scarce regarding specific Syndecan-4 partners involved in Thy-1/CD90-stimulated cell migration.

Methods

Mass spectrometry (MS) analysis of complexes precipitated with the Syndecan-4 cytoplasmic tail peptide was used to identify potential Syndecan-4-binding partners. The interactions found by MS were validated by immunoprecipitation and proximity ligation assays. The conducted research employed an array of genetic, biochemical and phar

SUBMITTER: Valdivia A 

PROVIDER: S-EPMC7433185 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature