Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa.

ABSTRACT: Chickens have been implicated in most Salmonella disease outbreaks because they act as carriers of the pathogen in their gut. There are over 2500 serotypes of Salmonella that have been reported worldwide and 2000 of these serovars can be found in chickens. The main objective of this study was to determine the Salmonella serotypes found in poultry farms around Mafikeng district, South Africa. Salmonella was identified according to the guidelines of the International Organization for Standardization (ISO) (ISO 6579:2002) standard techniques. Faecal samples were collected and analysed for Salmonella using conventional cultural methods and polymerase chain reaction targeting the 16S Ribosomal Deoxyribonucleic acid (rDNA) gene for Salmonella identification. Out of 130 presumptive Salmonella iso

SUBMITTER: Ramatla TA 

PROVIDER: S-EPMC7433231 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature