In Situ 3D-Printing using a Bio-ink of Protein-photosensitizer Conjugates for Single-cell Manipulation.

ABSTRACT: Living tissues dynamically modulate their structure and functions through physical and biochemical interactions in the three-dimensional (3D)-microenvironment for their homeostasis or the developmental process of an embryo. However, the manipulation of cellular functions in vitro is still challenging due to the lack of a dynamic material system that can vary the 3D-cellular microenvironment in time and space. Here, we show an in situ 3D-printing technique based on multiphoton lithography using a biocompatible photoresist, bio-ink. The bio-ink composed of protein-photosensitizer conjugates has the ability to cause singlet oxygen and cross-linking reaction to fabricate protein gels with submicrometer-scale precision. Remarkably, the conjugates substantially improve the cytocompatibility and

SUBMITTER: Nishiguchi A 

PROVIDER: S-EPMC7434051 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature