IL-17 production by tissue-resident MAIT cells is locally induced in children with pneumonia.

ABSTRACT: Community-acquired pneumonia (CAP) contributes substantially to morbidity and mortality in children under the age of 5 years. In examining bronchoalveolar lavages (BALs) of children with CAP, we found that interleukin-17 (IL-17) production was significantly increased in severe CAP. Immune profiling showed that mucosal-associated invariant T (MAIT) cells from the BALs, but not blood, of CAP patients actively produced IL-17 (MAIT17). Single-cell RNA-sequencing revealed that MAIT17 resided in a BAL-resident PLZF^hiCD103⁺ MAIT subset with high expression of hypoxia-inducible factor 1α (HIF-1α), reflecting the hypoxic state of the inflamed tissue. CAP BALs also contained a T-bet⁺ MAIT1 subset and a novel DDIT3⁺ (DNA damage-inducible transcript 3-positi

SUBMITTER: Lu B 

PROVIDER: S-EPMC7434594 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature