A novel strategy for promoting homoplasmic plastid transformant production using the barnase-barstar system.

ABSTRACT: Plastid transformants form biofactories that are able to produce extra proteins in plastids when they are in a homoplasmic state. To date, plastid transformation has been reported in about twenty plant species; however, the production of homoplasmic plastid transformants is not always successful or easy. Heteroplasmic plants that contain wild-type plastids produce fewer target proteins and do not always successfully transfer transgenes to progeny. In order to promote the generation of homoplasmic plants, we developed a novel system using barnase-barster to eliminate wild-type plastids from heteroplasmic cells systematically. In this system, a chemically inducible cytotoxic barnase under a plastid transit signal was introduced into nuclear DNA and barster, which inhibits barna

SUBMITTER: Okuzaki A 

PROVIDER: S-EPMC7434676 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature