Characterization of immune response induced against catalytic domain of botulinum neurotoxin type E.

ABSTRACT: Botulinum neurotoxins (BoNTs) represent a family of bacterial toxins responsible for neuroparalytic disease 'botulism' in human and animals. Their potential use as biological weapon led to their classification in category 'A' biowarfare agent by Centers for Disease Control and Prevention (CDC), USA. In present study, gene encoding full length catalytic domain of BoNT/E-LC was cloned, expressed and protein was purified using Ni-NTA chromatography. Humoral immune response was confirmed by Ig isotyping and cell-mediated immunity by cytokine profiling and intracellular staining for enumeration of IFN-γ secreting CD4⁺ and CD8⁺ T cells. Increased antibody titer with the predominance of IgG subtype was observed. An interaction between antibodies produced against rBoNT/E-LC w

SUBMITTER: Sonkar P 

PROVIDER: S-EPMC7434876 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature