Selection of a Full Agonist Combinatorial Antibody that Rescues Leptin Deficiency In Vivo.

ABSTRACT: Growth factor deficiency in adulthood constitutes a distinct clinical syndrome with significant morbidities including abnormal body composition, reduced energy, affective disturbances, dyslipidemia, and increased cardiovascular risk. Protein replacement therapies using recombinant proteins or enzymes represent the only approved treatment. Combinatorial antibodies have shown great promise as a new class of therapeutic molecules because they act as "mechanism-based antibodies" with both agonist and antagonist activities. Using leptin, a key hormone in energy metabolism, as an example, a function-guided approach is developed to select combinatorial antibodies with high potency and full agonist activity that substitute natural growth factors in vivo. The identified antibody shows identical bio

SUBMITTER: Tao P 

PROVIDER: S-EPMC7435230 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature