De novo assembly and characterization of the liver transcriptome of Mugil incilis (lisa) using next generation sequencing.

ABSTRACT: Mugil incilis (lisa) is an important commercial fish species in many countries, living along the coasts of the western Atlantic Ocean. It has been used as a model organism for environmental monitoring and ecotoxicological investigations. Nevertheless, available genomic and transcriptomic information for this organism is extremely deficient. The aim of this study was to characterize M. incilis hepatic transcriptome using Illumina paired-end sequencing. A total of 32,082,124 RNA-Seq read pairs were generated utilizing the HiSeq platform and subsequently cleaned and assembled into 93,912 contigs (N50 = 2,019 bp). The analysis of species distribution revealed that M. incilis contigs had the highest number of hits to Stegastes partitus (13.4%). Using a sequence similarity search against the pub

SUBMITTER: Bertel-Sevilla A 

PROVIDER: S-EPMC7435268 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature