Human-scale tissues with patterned vascular networks by additive manufacturing of sacrificial sugar-protein composites.

ABSTRACT: Combating necrosis, by supplying nutrients and removing waste, presents the major challenge for engineering large three-dimensional (3D) tissues. Previous elegant work used 3D printing with carbohydrate glass as a cytocompatible sacrificial template to create complex engineered tissues with vascular networks (Miller et al. 2012, Nature Materials). The fragile nature of this material compounded with the technical complexity needed to create high-resolution structures led us to create a flexible sugar-protein composite, termed Gelatin-sucrose matrix (GSM), to achieve a more robust and applicable material. Here we developed a low-range (25-37˚C) temperature sensitive formulation that can be moulded with micron-resolution features or cast during 3D printing to produce complex flexible filament

SUBMITTER: Eltaher HM 

PROVIDER: S-EPMC7435351 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature